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Various constructs were used for mammalian cell assays including human GLP-1R in pcDNA3.1 modified with a 2xcMyc-N-terminal epitope tag, and human GLP-1R with a c-terminal Rluc-8 tag

Structural alignment of the GSH-bound OsDHAR (C20S mutant), hCLIC1 (PDB code 1K0N

In animal models, the rs1275988C/C genotype, combined with a high-salt diet, exacerbates hypertension and induces pronounced vascular remodeling

Low grade inflammation common to maternal obesity may induce effects in humans more similar to the inflammation animal models, leading to a higher rate of maternal -fetal transfer and direct effects in the fetus

3 Evidence summary